Review



goat anti mouse cd31 primary antibody  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 98
      Buy from Supplier

    Structured Review

    R&D Systems goat anti mouse cd31 primary antibody
    Superior SA node myocytes exhibit elevated diastolic ATP and metabolic flux compared with the inferior region. (A) 3D segmented maximum-intensity projection of a whole-mount SA node immunolabeled for <t>CD31</t> (vasculature, red) and cyto-iATP (myocytes, green). The dashed line denotes the boundary between superior and inferior regions. (B) Image-processing workflow illustrating merged maximum-intensity projections, binary segmentation masks, and extraction of grayscale cyto-iATP signals used for quantitative analysis. (C) Mean cyto-iATP fluorescence intensity per myocyte, grouped by region ( N = 5 mice per region), reporting expression levels of the EGFP-tagged cyto-iATP sensor. (D) Live confocal imaging of cyto-iATP signals showing representative line-scan images and corresponding normalized fluorescence traces (F/F 0 ) from superior and inferior regions. (E and F) Summary quantification of cyto-iATP signal mass rate (E) and estimated diastolic [ATP] i (F). P values are shown above comparisons. Large circles denote per-animal means; small circles indicate individual biological replicates. N represents the number of independent mice.
    Goat Anti Mouse Cd31 Primary Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1273 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+mouse+cd31+primary+antibody/Human%2FMouse%2FRat+CD31%2FPECAM-1+Antibody/pmc13037587-53-13-20
    Average 98 stars, based on 1273 article reviews
    goat anti mouse cd31 primary antibody - by Bioz Stars, 2026-10
    98/100 stars

    Images

    1) Product Images from "Beat-locked ATP microdomains in the sinoatrial node map a Ca 2+ -timed energetic hierarchy and regional pacemaker roles"

    Article Title: Beat-locked ATP microdomains in the sinoatrial node map a Ca 2+ -timed energetic hierarchy and regional pacemaker roles

    Journal: The Journal of General Physiology

    doi: 10.1085/jgp.202513874

    Superior SA node myocytes exhibit elevated diastolic ATP and metabolic flux compared with the inferior region. (A) 3D segmented maximum-intensity projection of a whole-mount SA node immunolabeled for CD31 (vasculature, red) and cyto-iATP (myocytes, green). The dashed line denotes the boundary between superior and inferior regions. (B) Image-processing workflow illustrating merged maximum-intensity projections, binary segmentation masks, and extraction of grayscale cyto-iATP signals used for quantitative analysis. (C) Mean cyto-iATP fluorescence intensity per myocyte, grouped by region ( N = 5 mice per region), reporting expression levels of the EGFP-tagged cyto-iATP sensor. (D) Live confocal imaging of cyto-iATP signals showing representative line-scan images and corresponding normalized fluorescence traces (F/F 0 ) from superior and inferior regions. (E and F) Summary quantification of cyto-iATP signal mass rate (E) and estimated diastolic [ATP] i (F). P values are shown above comparisons. Large circles denote per-animal means; small circles indicate individual biological replicates. N represents the number of independent mice.
    Figure Legend Snippet: Superior SA node myocytes exhibit elevated diastolic ATP and metabolic flux compared with the inferior region. (A) 3D segmented maximum-intensity projection of a whole-mount SA node immunolabeled for CD31 (vasculature, red) and cyto-iATP (myocytes, green). The dashed line denotes the boundary between superior and inferior regions. (B) Image-processing workflow illustrating merged maximum-intensity projections, binary segmentation masks, and extraction of grayscale cyto-iATP signals used for quantitative analysis. (C) Mean cyto-iATP fluorescence intensity per myocyte, grouped by region ( N = 5 mice per region), reporting expression levels of the EGFP-tagged cyto-iATP sensor. (D) Live confocal imaging of cyto-iATP signals showing representative line-scan images and corresponding normalized fluorescence traces (F/F 0 ) from superior and inferior regions. (E and F) Summary quantification of cyto-iATP signal mass rate (E) and estimated diastolic [ATP] i (F). P values are shown above comparisons. Large circles denote per-animal means; small circles indicate individual biological replicates. N represents the number of independent mice.

    Techniques Used: Immunolabeling, Extraction, Fluorescence, Expressing, Imaging

    Related Articles

    Incubation:

    Article Title: The Alzheimer risk factor CD2AP causes dysfunction of the brain vascular network
    Article Snippet: Stained slides were returned to Genentech for imaging and quantitation; unused sections were returned to Genentech for cryoprotected storage until used for additional stains. .. For CD31 stains, sections were washed in PBS and then PBS plus Triton X-100 (PBST, 0.1%) and then blocked in PBST (0.3%) with 5% BSA and 5% normal donkey serum, then incubated overnight with goat anti-mouse CD31 primary antibody (10 μg/mL; R&D Systems) diluted in PBST (0.3%) plus 1% BSA at 4°C. .. Primary antibody incubation was followed by three 10 min washes in PBST, followed by incubation with a secondary antibody (donkey anti-goat IgG-Alexa555; 1:500; Thermo Fisher Scientific) for 2 h at room temperature.

    Article Title: Beat-locked ATP microdomains in the sinoatrial node map a Ca 2+ -timed energetic hierarchy and regional pacemaker roles
    Article Snippet: .. For immunolabeling, SA nodes were incubated for 48 h at 4°C with a goat anti-mouse CD31 primary antibody (1:50, AF3628; R&D Systems). .. After three 10-min PBS washes, tissues were incubated for 4 h at room temperature in the dark with donkey anti-goat Alexa Fluor 568 (1:1,000, A11057; Thermo Fisher Scientific) and/or anti-GFP Alexa Fluor 647 (1:1,000, A32985; Thermo Fisher Scientific) secondary antibodies.

    Article Title: Beat-locked ATP microdomains in the sinoatrial node map a Ca 2+ -timed energetic hierarchy and regional pacemaker roles
    Article Snippet: .. For immunolabeling, SA nodes were incubated at 4°C for 48 hours with goat anti-mouse CD31 primary antibody (AF3628; R&D Systems), diluted 1:50 in PBS. .. After three PBS washes (10 minutes each), tissues were incubated for 4 hours at room temperature in the dark with donkey anti-goat Alexa Fluor 568 (1:1000, A11057; Thermo Fisher Scientific) or anti-GFP Alexa Fluor 647 (1:1000, A32985; Thermo Fisher Scientific) secondary antibodies.

    Article Title: Safety, Distribution, and Pharmacokinetics of Biodegradable P(AAm-co-MAA) Nanogels Following Systemic Administration in Mice.
    Article Snippet: Systematic analysis of the fate of hydrogel nanoparticles after in vivo administration is essential for their clinical translation.. Biodegradable, disulfidecrosslinked synthetic nanogels are a promising platform for the delivery of therapeutic molecules, but their biodistribution and clearance profiles remain underexplored compared to other solid nanoparticles.. In this study, we investigated the safety, pharmacokinetics, tissue, and cellular distribution profiles of poly(acrylamidecomethacrylic acid) (P(AAmcoMAA)) nanogels following a single intravenous or intraperitoneal injection.

    Article Title: Beat-locked ATP microdomains in the sinoatrial node map a Ca2+-timed energetic hierarchy and regional pacemaker roles.
    Article Snippet: .. For immunolabeling, SA nodes were incubated for 48 h at 4°C with a goat anti-mouse CD31 primary antibody (1:50, AF3628; R&D Systems). .. After three 10-min PBS washes, tissues were incubated for 4 h at room temperature in the dark with donkey anti-goat Alexa Fluor 568 (1:1,000, A11057; Thermo Fisher Scientific) and/or anti-GFP Alexa Fluor 647 (1: 1,000, A32985; Thermo Fisher Scientific) secondary antibodies.

    Immunolabeling:

    Article Title: Beat-locked ATP microdomains in the sinoatrial node map a Ca 2+ -timed energetic hierarchy and regional pacemaker roles
    Article Snippet: .. For immunolabeling, SA nodes were incubated for 48 h at 4°C with a goat anti-mouse CD31 primary antibody (1:50, AF3628; R&D Systems). .. After three 10-min PBS washes, tissues were incubated for 4 h at room temperature in the dark with donkey anti-goat Alexa Fluor 568 (1:1,000, A11057; Thermo Fisher Scientific) and/or anti-GFP Alexa Fluor 647 (1:1,000, A32985; Thermo Fisher Scientific) secondary antibodies.

    Article Title: Beat-locked ATP microdomains in the sinoatrial node map a Ca 2+ -timed energetic hierarchy and regional pacemaker roles
    Article Snippet: .. For immunolabeling, SA nodes were incubated at 4°C for 48 hours with goat anti-mouse CD31 primary antibody (AF3628; R&D Systems), diluted 1:50 in PBS. .. After three PBS washes (10 minutes each), tissues were incubated for 4 hours at room temperature in the dark with donkey anti-goat Alexa Fluor 568 (1:1000, A11057; Thermo Fisher Scientific) or anti-GFP Alexa Fluor 647 (1:1000, A32985; Thermo Fisher Scientific) secondary antibodies.

    Article Title: Beat-locked ATP microdomains in the sinoatrial node map a Ca2+-timed energetic hierarchy and regional pacemaker roles.
    Article Snippet: .. For immunolabeling, SA nodes were incubated for 48 h at 4°C with a goat anti-mouse CD31 primary antibody (1:50, AF3628; R&D Systems). .. After three 10-min PBS washes, tissues were incubated for 4 h at room temperature in the dark with donkey anti-goat Alexa Fluor 568 (1:1,000, A11057; Thermo Fisher Scientific) and/or anti-GFP Alexa Fluor 647 (1: 1,000, A32985; Thermo Fisher Scientific) secondary antibodies.



    Similar Products

    human/mouse/rat cd31/pecam-1 antibody  (Bio-Techne corporation)
    99
    Bio-Techne corporation human/mouse/rat cd31/pecam-1 antibody
    Human/Mouse/Rat Cd31/Pecam 1 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+mouse+cd31+primary+antibody/Human%2FMouse%2FRat+CD31%2FPECAM-1+Antibody/custom%40af3628%4041794795
    Average 99 stars, based on 1 article reviews
    human/mouse/rat cd31/pecam-1 antibody - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    goat anti mouse cd31 primary antibody  (R&D Systems)
    98
    R&D Systems goat anti mouse cd31 primary antibody
    Superior SA node myocytes exhibit elevated diastolic ATP and metabolic flux compared with the inferior region. (A) 3D segmented maximum-intensity projection of a whole-mount SA node immunolabeled for <t>CD31</t> (vasculature, red) and cyto-iATP (myocytes, green). The dashed line denotes the boundary between superior and inferior regions. (B) Image-processing workflow illustrating merged maximum-intensity projections, binary segmentation masks, and extraction of grayscale cyto-iATP signals used for quantitative analysis. (C) Mean cyto-iATP fluorescence intensity per myocyte, grouped by region ( N = 5 mice per region), reporting expression levels of the EGFP-tagged cyto-iATP sensor. (D) Live confocal imaging of cyto-iATP signals showing representative line-scan images and corresponding normalized fluorescence traces (F/F 0 ) from superior and inferior regions. (E and F) Summary quantification of cyto-iATP signal mass rate (E) and estimated diastolic [ATP] i (F). P values are shown above comparisons. Large circles denote per-animal means; small circles indicate individual biological replicates. N represents the number of independent mice.
    Goat Anti Mouse Cd31 Primary Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+mouse+cd31+primary+antibody/Human%2FMouse%2FRat+CD31%2FPECAM-1+Antibody/pmc13037587-53-13-20
    Average 98 stars, based on 1 article reviews
    goat anti mouse cd31 primary antibody - by Bioz Stars, 2026-10
    98/100 stars
    		  Buy from Supplier
    polyclonal goat anti cd31 primary antibody  (R&D Systems)
    98
    R&D Systems polyclonal goat anti cd31 primary antibody
    Superior SA node myocytes exhibit elevated diastolic ATP and metabolic flux compared with the inferior region. (A) 3D segmented maximum-intensity projection of a whole-mount SA node immunolabeled for <t>CD31</t> (vasculature, red) and cyto-iATP (myocytes, green). The dashed line denotes the boundary between superior and inferior regions. (B) Image-processing workflow illustrating merged maximum-intensity projections, binary segmentation masks, and extraction of grayscale cyto-iATP signals used for quantitative analysis. (C) Mean cyto-iATP fluorescence intensity per myocyte, grouped by region ( N = 5 mice per region), reporting expression levels of the EGFP-tagged cyto-iATP sensor. (D) Live confocal imaging of cyto-iATP signals showing representative line-scan images and corresponding normalized fluorescence traces (F/F 0 ) from superior and inferior regions. (E and F) Summary quantification of cyto-iATP signal mass rate (E) and estimated diastolic [ATP] i (F). P values are shown above comparisons. Large circles denote per-animal means; small circles indicate individual biological replicates. N represents the number of independent mice.
    Polyclonal Goat Anti Cd31 Primary Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+mouse+cd31+primary+antibody/Human%2FMouse%2FRat+CD31%2FPECAM-1+Antibody/pm41616887-158-17-22
    Average 98 stars, based on 1 article reviews
    polyclonal goat anti cd31 primary antibody - by Bioz Stars, 2026-10
    98/100 stars
    		  Buy from Supplier
    primary goat anti mouse cd31  (R&D Systems)
    93
    R&D Systems primary goat anti mouse cd31
    Superior SA node myocytes exhibit elevated diastolic ATP and metabolic flux compared with the inferior region. (A) 3D segmented maximum-intensity projection of a whole-mount SA node immunolabeled for <t>CD31</t> (vasculature, red) and cyto-iATP (myocytes, green). The dashed line denotes the boundary between superior and inferior regions. (B) Image-processing workflow illustrating merged maximum-intensity projections, binary segmentation masks, and extraction of grayscale cyto-iATP signals used for quantitative analysis. (C) Mean cyto-iATP fluorescence intensity per myocyte, grouped by region ( N = 5 mice per region), reporting expression levels of the EGFP-tagged cyto-iATP sensor. (D) Live confocal imaging of cyto-iATP signals showing representative line-scan images and corresponding normalized fluorescence traces (F/F 0 ) from superior and inferior regions. (E and F) Summary quantification of cyto-iATP signal mass rate (E) and estimated diastolic [ATP] i (F). P values are shown above comparisons. Large circles denote per-animal means; small circles indicate individual biological replicates. N represents the number of independent mice.
    Primary Goat Anti Mouse Cd31, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+mouse+cd31+primary+antibody/Mouse+CD31%2FPECAM-1+Biotinylated+Antibody/us12378329-551-16-20
    Average 93 stars, based on 1 article reviews
    primary goat anti mouse cd31 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    primary antibody goat anti mouse cd31  (R&D Systems)
    98
    R&D Systems primary antibody goat anti mouse cd31
    Superior SA node myocytes exhibit elevated diastolic ATP and metabolic flux compared with the inferior region. (A) 3D segmented maximum-intensity projection of a whole-mount SA node immunolabeled for <t>CD31</t> (vasculature, red) and cyto-iATP (myocytes, green). The dashed line denotes the boundary between superior and inferior regions. (B) Image-processing workflow illustrating merged maximum-intensity projections, binary segmentation masks, and extraction of grayscale cyto-iATP signals used for quantitative analysis. (C) Mean cyto-iATP fluorescence intensity per myocyte, grouped by region ( N = 5 mice per region), reporting expression levels of the EGFP-tagged cyto-iATP sensor. (D) Live confocal imaging of cyto-iATP signals showing representative line-scan images and corresponding normalized fluorescence traces (F/F 0 ) from superior and inferior regions. (E and F) Summary quantification of cyto-iATP signal mass rate (E) and estimated diastolic [ATP] i (F). P values are shown above comparisons. Large circles denote per-animal means; small circles indicate individual biological replicates. N represents the number of independent mice.
    Primary Antibody Goat Anti Mouse Cd31, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+mouse+cd31+primary+antibody/Human%2FMouse%2FRat+CD31%2FPECAM-1+Antibody/pm38052996-423-33-38
    Average 98 stars, based on 1 article reviews
    primary antibody goat anti mouse cd31 - by Bioz Stars, 2026-10
    98/100 stars
    		  Buy from Supplier
    primary if goat anti mouse cd31 r d systems  (R&D Systems)
    98
    R&D Systems primary if goat anti mouse cd31 r d systems
    Superior SA node myocytes exhibit elevated diastolic ATP and metabolic flux compared with the inferior region. (A) 3D segmented maximum-intensity projection of a whole-mount SA node immunolabeled for <t>CD31</t> (vasculature, red) and cyto-iATP (myocytes, green). The dashed line denotes the boundary between superior and inferior regions. (B) Image-processing workflow illustrating merged maximum-intensity projections, binary segmentation masks, and extraction of grayscale cyto-iATP signals used for quantitative analysis. (C) Mean cyto-iATP fluorescence intensity per myocyte, grouped by region ( N = 5 mice per region), reporting expression levels of the EGFP-tagged cyto-iATP sensor. (D) Live confocal imaging of cyto-iATP signals showing representative line-scan images and corresponding normalized fluorescence traces (F/F 0 ) from superior and inferior regions. (E and F) Summary quantification of cyto-iATP signal mass rate (E) and estimated diastolic [ATP] i (F). P values are shown above comparisons. Large circles denote per-animal means; small circles indicate individual biological replicates. N represents the number of independent mice.
    Primary If Goat Anti Mouse Cd31 R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+mouse+cd31+primary+antibody/Human%2FMouse%2FRat+CD31%2FPECAM-1+Antibody/pm38799448-78-220-226
    Average 98 stars, based on 1 article reviews
    primary if goat anti mouse cd31 r d systems - by Bioz Stars, 2026-10
    98/100 stars
    		  Buy from Supplier

    Image Search Results


    Superior SA node myocytes exhibit elevated diastolic ATP and metabolic flux compared with the inferior region. (A) 3D segmented maximum-intensity projection of a whole-mount SA node immunolabeled for CD31 (vasculature, red) and cyto-iATP (myocytes, green). The dashed line denotes the boundary between superior and inferior regions. (B) Image-processing workflow illustrating merged maximum-intensity projections, binary segmentation masks, and extraction of grayscale cyto-iATP signals used for quantitative analysis. (C) Mean cyto-iATP fluorescence intensity per myocyte, grouped by region ( N = 5 mice per region), reporting expression levels of the EGFP-tagged cyto-iATP sensor. (D) Live confocal imaging of cyto-iATP signals showing representative line-scan images and corresponding normalized fluorescence traces (F/F 0 ) from superior and inferior regions. (E and F) Summary quantification of cyto-iATP signal mass rate (E) and estimated diastolic [ATP] i (F). P values are shown above comparisons. Large circles denote per-animal means; small circles indicate individual biological replicates. N represents the number of independent mice.

    Journal: The Journal of General Physiology

    Article Title: Beat-locked ATP microdomains in the sinoatrial node map a Ca 2+ -timed energetic hierarchy and regional pacemaker roles

    doi: 10.1085/jgp.202513874

    Figure Lengend Snippet: Superior SA node myocytes exhibit elevated diastolic ATP and metabolic flux compared with the inferior region. (A) 3D segmented maximum-intensity projection of a whole-mount SA node immunolabeled for CD31 (vasculature, red) and cyto-iATP (myocytes, green). The dashed line denotes the boundary between superior and inferior regions. (B) Image-processing workflow illustrating merged maximum-intensity projections, binary segmentation masks, and extraction of grayscale cyto-iATP signals used for quantitative analysis. (C) Mean cyto-iATP fluorescence intensity per myocyte, grouped by region ( N = 5 mice per region), reporting expression levels of the EGFP-tagged cyto-iATP sensor. (D) Live confocal imaging of cyto-iATP signals showing representative line-scan images and corresponding normalized fluorescence traces (F/F 0 ) from superior and inferior regions. (E and F) Summary quantification of cyto-iATP signal mass rate (E) and estimated diastolic [ATP] i (F). P values are shown above comparisons. Large circles denote per-animal means; small circles indicate individual biological replicates. N represents the number of independent mice.

    Article Snippet: For immunolabeling, SA nodes were incubated for 48 h at 4°C with a goat anti-mouse CD31 primary antibody (1:50, AF3628; R&D Systems).

    Techniques: Immunolabeling, Extraction, Fluorescence, Expressing, Imaging